ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Detail ...

Study Report

Basic Info

Reference	Laurin N, 2008(a)18081024
Citation	Laurin N., Lee J., Ickowicz A., Pathare T., Malone M., Tannock R., Kennedy J. L., Schachar R. J. and Barr C. L. (2008) "Association study for genes at chromosome 5p13-q11 in attention deficit hyperactivity disorder." Am J Med Genet B Neuropsychiatr Genet, 147B(5): 600-5.
Study Design	family-based
Study Type	Candidate-gene association study
Sample Size	245 nuclear families
Predominant Ethnicity	Caucasian
Population	Canada
Gender	224 boys and 56 girls
Age Group	Children/Adolescents : 7-16 years

Detail Info

Summary	The aim of this study was to investigate some of the top candidates among these genes in relation to ADHD in a sample of 245 nuclear families from the Toronto area. They investigated the genes for the glial cell-derived neurotropic factor (GDNF), the fibroblast growth factor 10 (FGF10), islet-1 (ISL1), the hyperpolarized potassium channel (HCN1) and the integrin alpha 1 (ITGA1). In addition to these genes, they assessed the 3'region of the SLC1A3 gene, a glutamate transporter implicated in ADHD by a previous association study. A total of 36 polymorphisms were selected across the six genes. They performed family-based association and haplotype analyses. Single marker and haplotype analyses yielded little evidence of association for any of the genes tested in this study. Moreover, they were unable to replicate the positive association findings reported for SLC1A3. Their results suggest that these six genes are unlikely to be susceptibility genes in the chromosome 5p13-q11 region and other genes should now be considered for priority study.
Total Sample	The study sample was comprised of 245 nuclear families from the Toronto area, including 35 affected siblings. This gave a total of 280 affected children (224 boys and 56 girls). The sample consists of 179 parents-child trios and 66 families in which a single parent was genotyped.
Sample Collection	The majority of the families reported their ethnic background to be of European Caucasian descent, while 10% of families were of other or mixed background, including Chinese, African, Indian, and Native-Canadians.
Diagnosis Description	The methods of assessment, characteristics of the subjects, and inclusion/exclusion criteria have been described previously, including the instruments used to collect information for the diagnosis of ADHD and co-morbid conditions [Quist et al., 2000; Laurin et al., 2005]. Briefly, probands and their siblings between 7 and 16 years old were included if they met DSM-IV criteria for one of the three ADHD subtypes (predominantly inattentive, predominantly hyperactive/ impulsive, or combined). The distribution of the affected children among the DSM-IV ADHD subtypes was 14% of the predominantly hyperactive/impulsive subtype, 24% of the predominantly inattentive subtype and 62% of the combined subtype.
Technique	A total of 36 SNPs were genotyped in this study. DNA was isolated from blood lymphocytes using a standard high salt extraction method. SNPs were genotyped using the TaqMan 5'nuclease assays for allelic discrimination implemented on a ABI 7900-HT Sequence Detection System1 as described previously [Feng et al., 2005; Laurin et al., 2005].
Analysis Method	For the analysis of ADHD as a categorical trait, the PDTphase program from the UNPHASED package was used to test for biased transmission of individual marker alleles while the TRANSMIT program was used for the haplotype analysis, with the robust estimator of variance (-ro option).
Result Description	Single marker and haplotype analyses yielded little evidence of association for any of the genes tested in this study. Moreover, they were unable to replicate the positive association findings reported for SLC1A3.

SNPs reported by this study (count: 36)

SNP	Allele Change	Statistical Values	Author Comments	Result of Statistical Analysis
rs3776581	G/A	P-value=0.574 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2406217	C/G	P-value=0.38 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs3812047	C/T	P-value=0.354 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2406369	G/A	P-value=0.663 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs7722380	G/C	P-value=0.253 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2456205	G/T	P-value=0.366 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs7731209	C/T	P-value=0.747 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2589162	A/G	P-value=1 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2860025	G/A	P-value=0.333 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2216711	A/G	P-value=0.394 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2910797	C/T	P-value=0.733 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2269272	C/T	P-value=0.963 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2973041	T/C	P-value=0.946 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2288648	T/C	P-value=0.496 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2973050	G/A	P-value=0.792 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2290070	C/G	P-value=0.56 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs16902086	A/G	P-value=0.706 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2032893	C/T	P-value=0.746 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2126953	T/G	P-value=0.078 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs2193968	A/G	P-value=0.844 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs7732839	A/T	P-value=0.12 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs1421927	A/G	P-value=0.877 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs980510	C/A	P-value=0.619 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs1478438	T/A	P-value=0.536 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs994092	T/C	P-value=0.783 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs1478442	A/G	P-value=0.485 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs1549250	C/A	P-value=0.643 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs12518844	G/A	P-value=0.852 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs12110170	T/A	P-value=0.928 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs1391983	T/A	P-value=0.336 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs13188662	A/G	P-value=0.16 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs11743392	T/C	P-value=0.645 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs10513003	C/T	P-value=0.097 in the catergorical analysis, PICS inat P-value=0.013, PICS HI P-value=0.008 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs11954894	C/T	P-value=0.079 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs11743802	T/C	P-value=0.967 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant
rs10491412	C/T	P-value=0.438 in the catergorical analysis, P-value>0.05 in the quantitative analysis	no significant evidence of association no significant evidence of association	Non-significant

Genes reported by this study (count: 6)

Gene	Statistical Values/Author Comments	Result of Statistical Analysis
ITGA1	single marker and haplotype analyses yielded little evidence...... single marker and haplotype analyses yielded little evidence for significant association More...	Non-significant
GDNF	two low frequency haplotypes demonstrated marginal evidence ...... two low frequency haplotypes demonstrated marginal evidence of association (P-value=0.011 and P-value=0.049), but not supported by the quantitative analyses More...	Significant
FGF10	single marker and haplotype analyses yielded little evidence...... single marker and haplotype analyses yielded little evidence for significant association More...	Non-significant
HCN1	single marker and haplotype analyses yielded little evidence...... single marker and haplotype analyses yielded little evidence for significant association More...	Non-significant
SLC1A3	single marker and haplotype analyses yielded little evidence...... single marker and haplotype analyses yielded little evidence for significant association More...	Non-significant
ISL1	single marker and haplotype analyses yielded little evidence...... single marker and haplotype analyses yielded little evidence for significant association More...	Non-significant