ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Renner TJ, 200818250960
Citation	Renner T. J., Walitza S., Dempfle A., Eckert L., Romanos M., Gerlach M., Schafer H., Warnke A., Lesch K. P. and Jacob C. (2008) "Allelic variants of SNAP25 in a family-based sample of ADHD." J Neural Transm, 115(2): 317-21.
Study Design	family-based
Study Type	Candidate-gene association study
Sample Size	161 children with ADHD from 111 families were recruited, but 102 families with 151 affected children were genotyped successfully
Predominant Ethnicity	Caucasian
Population	Germany
Gender	129 males and 36 females
Age Group	Children/Adolescents : 11.9 years (SD=3.6 years)

Detail Info

Summary	In this study they aimed to investigate association of genetic variants of SNAP25 located in the putative promoter region of SNAP25 and a SNP in intron 8, previously reported to associated with ADHD. A family based design was applied to detect preferential transmission of genetic variants. In their German ADHD sample no preferential transmission of either variant could be observed. Further investigation considering sub-sample analysis regarding response to D-amphetamine could enlight the role of SNAP25 in ADHD.
Total Sample	One hundred and sixty-one children with ADHD from 111 families were recruited and phenotypically characterized by a team of experienced psychiatrists in the outpatient unit of the Department of Child and Adolescent Psychiatry and Psychotherapy, University of Wurzburg according DSM-IV criteria. The sample includes 129 males (78.2%) and 36 females (21.8%). In 72 families one child, in 26 families two, in 11 families three and in 2 families four affected children were recruited. Of the recruited sample 102 families with 151 affected children were finally included in the statistical analysis, 9 families dropped out due technical problems in genotyping.
Diagnosis Description	The index patient was required to be older than 8 years and to fulfil DSM-IV criteria for the combined subtype, other affected siblings in a family had to be older than 6 years. The lower limit was chosen in order to ensure relative persistence of ADHD symptoms and to exclude children who may show phenocopies of the disorder during preschool age but do not fulfil diagnostic criteria for ADHD during subsequent developmental stages. Please refer to the original article for more details.
Technique	DNA was extracted out of whole blood samples according to standard protocols. Genotyping for was performed via PCR, enzyme digestion and subsequent gel electrophoresis. Primers were designed by using FastPCR and Autodimer, respectively.
Analysis Method	A family-based association test which is valid for families with an arbitrary number of affected children was performed for three SNPs in SNAP25 by using a permutation test as implemented in FAMHAP. Analysis of linkage disequilibrium (LD) between the investigated SNAP25 polymorphisms was also done with the program FAMHAP.
Result Description	No significant preferential transmissions to affected children with ADHD were detected. Haplotype analyses also did not show any significant transmission disequilibrium. The global P-value corrected for multiple testing for the haplotype analysis of all marker combinations in SNAP25 was P-value=0.89.

SNPs reported by this study (count: 3)

SNP	Allele Change	Risk Allele	Statistical Values	Author Comments	Result of Statistical Analysis
rs363006			FAMHAP P-value=1	no significant preferential transmissions to affected childr...... no significant preferential transmissions to affected children with ADHD were detected More...	Non-significant
rs6077690			FAMHAP P-value=0.86	no significant preferential transmissions to affected childr...... no significant preferential transmissions to affected children with ADHD were detected More...	Non-significant
rs6039769			FAMHAP P-value=0.54	no significant preferential transmissions to affected childr...... no significant preferential transmissions to affected children with ADHD were detected More...	Non-significant

Genes reported by this study (count: 1)