ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Friedel S, 200515457498
Citation	Friedel S., Horro F. F., Wermter A. K., Geller F., Dempfle A., Reichwald K., Smidt J., Bronner G., Konrad K., Herpertz-Dahlmann B., Warnke A., Hemminger U., Linder M., Kiefl H., Goldschmidt H. P., Siegfried W., Remschmidt H., Hinney A. and Hebebrand J. (2005) "Mutation screen of the brain derived neurotrophic factor gene (BDNF): identification of several genetic variants and association studies in patients with obesity, eating disorders, and attention-deficit/hyperactivity disorder." Am J Med Genet B Neuropsychiatr Genet, 132B(1): 96-9.
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	88 patients with ADHD, and 96 normal weight controls
Predominant Ethnicity	Caucasian
Population	Germany
Age Group	Children/Adolescents

Detail Info

Summary	A mutation screen (SSCP and DHPLC) of the translated region of BDNF in 183 extremely obese children and adolescents and 187 underweight students was performed. Additionally, they genotyped two common polymorphisms (rs6265: p.V66M; c.-46C>T) in 118 patients with anorexia nervosa, 80 patients with bulimia nervosa, 88 patients with ADHD, and 96 normal weight controls. Three rare variants (c.5C>T: p.T2I; c.273G>A; c.*137A>G) and the known polymorphism (p.V66M) were identified. A role of the I2 allele in the etiology of obesity cannot be excluded. They found no association between p.V66M or the additionally genotyped variant c.-46C>T and obesity, ADHD or eating disorders.
Total Sample	They analyzed two common polymorphisms (V66M in the 5' pro-region; SNP c.-46C>T in one of the 5'UTR exons, Kunugi et al., 2001) in the initial study groups and additionally in 118 patients with AN, 80 patients with BN, 88 patients with ADHD, and 96 normal weight controls.
Sample Collection	For association studies, they used the initial screening-sample and samples of patients with ADHD, AN, BN, and normal weight controls (see the online Table II at http://www.interscience. wiley.com/jpages/0148-7299:1/suppmat/index.html).
Diagnosis Description	Patients with ED or with ADHD fulfilled DSM-IV criteria [APA, 1994]. Written informed consent was given by all participants and, in the case of minors, their parents. This study was approved by the Ethics Committee of the University of Marburg.
Technique	PCR products of all SNPs were run on ethidium bromide-stained 2.5% agarose gels. Positive controls for the variant alleles were run on each gel. For validity of the genotypes, allele determinations were rated independently by at least two experienced individuals. Discrepancies were resolved unambiguously either by reaching consensus or by retyping (see the online Table IV at http://www.interscience.wiley.com/jpages/ 0148-7299:1/suppmat/index.html).
Analysis Method	Differences in genotype frequencies were investigated using the Cochran-Armitage trend test. Pearson's X²-tests were carried out to investigate differences in allele frequencies. They did not correct for the multiple tests they performed for the different groups at the two loci. Therefore, all reported P values are nominal.
Result Description	Association studies revealed no significant differences in genotype or allele distributions between extremely obese children and adolescents and underweight controls, as well as between AN,ADHD, and normal weight students; all nominal P values were>0.2.

SNPs reported by this study (count: 1)

Other variant reported by this study (count: 1)

Genes reported by this study (count: 1)