ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Li J, 200515578608
Citation	Li J., Wang Y., Zhou R., Zhang H., Yang L., Wang B., Khan S. and Faraone S. V. (2005) "Serotonin 5-HT1B receptor gene and attention deficit hyperactivity disorder in Chinese Han subjects." Am J Med Genet B Neuropsychiatr Genet, 132B(1): 59-63.
Study Design	family-based
Study Type	Candidate-gene association study
Sample Size	358 unrelated complete trios
Predominant Ethnicity	Mongoloid
Population	China
Gender	295 (82.4%) male probands and 63 (17.6%) female probands
Age Group	Children/Adolescents : 6-17 years, mean age: 10.5, SD=2.5

Detail Info

Summary	Prior studies reported excess transmission of the 861G allele of 5-HT1B to ADHD offspring. They used the transmission disequilibrium test (TDT) and haplotype analysis to investigate the A-161T and G861C polymorphisms in the 5-HT1B receptor gene in ADHD trios from the Chinese Han population. They found no association with ADHD but did find a tendency for excess transmission of the 861G allele and the G/A haplotype, and under-transmission of C/A haplotype to offspring with inattentive ADHD.
Total Sample	A total of 358 unrelated complete trios, composed of ADHD probands and both biological parents were recruited. All non-Han families were excluded. The age of the probands ranged from 6 to 17 years, with a mean age of 10.5 (SD=2.5) years. The gender composition of the sample included 295 (82.4%) male probands and 63 (17.6%) female probands.
Sample Collection	A total of 358 unrelated complete trios, composed of ADHD probands and both biological parents were recruited from the child psychiatric clinics at Peking University Institute of Mental Health from September 1999 to April 2003.
Diagnosis Description	Diagnoses of ADHD and subtypes of ADHD were based on the USAn clinical diagnostic interview scales (CDIS) [Barkley, 1998], a structured interview derived from the DSM-IV. Two psychiatrists, one of who was of senior status, interviewed all parents separately. Additionally, teachers completed Rutter's scale to evaluate the children's behaviors at school. Based upon data collected from the CDIS, parent interviews and teacher reports, consensus diagnoses were assigned to all probands. Of the 358 ADHD trios, 146 (40.8%) probands met criteria for ADHD-C, 23 (6.4%) met criteria for ADHD-HI, and 187 (52.3%) met criteria for ADHD-I.
Technique	G861C Polymorphism Typing: Forward primer 5'-AGG ACA GAC GCC CAA CAG GAC -3' and reverse primer 5'-CCA GAA ACC GCG AAA GAA GAT -3' were used to generate 548 bp fragments of G861C polymorphism. A 25 ul reaction was performed according to the protocol previously described [Lappalainen et al., 1995] on a PE-9700 using a PE-2400 thermal cycler (Perkin Elmer Company, Branchburg, New Jersey, USA). A-161T Polymorphism Typing: Forward primer 5'-GTT CCT CCA TGG CTC TCC TCG-3' and reverse primer 5'-CAG CGC TGC TCC TAG ACT TCA CC-3' were used to generate 605 bp fragments of A-161T polymorphism. A25 ul reaction was performed according to the protocol previously described [Sun et al., 2002]. More descriptions about the laboratory procedures could be found in the original publication.
Analysis Method	The TDT was performed to explore the possible linkage and association of the two polymorphisms with ADHD. In addition, they also performed haplotype analysis by using the TRANSMIT program [version 2.5; Clayton, 1999].
Result Description	They found no association with ADHD but did find a tendency for excess transmission of the 861G allele (x²=3.766, P=0.052) and the G/A haplotype (x²=2.925, df=1, P=0.087), and under-transmission of C/A haplotype (x²=3.707, df=1, P=0.054) to offspring with inattentive ADHD.

Other variant reported by this study (count: 2)

Variant Name	Allele Change	Risk Allele	Statistical Values	Author Comments	Result of Statistical Analysis
HTR1B G861C	C/G		allelic TDT P-value=0.413, McNemar X²=0.669 in AD...... allelic TDT P-value=0.413, McNemar X²=0.669 in ADHD; allelic TDT P-value=0.27, McNemar X²=1.217 in ADHD combined type; allelic TDT P-value=0.052, McNemar X²=3.766 in ADHD inattentive type More...	no biased transmission was observed in families of ADHD probands; a preferential transmission of G allele was observed in ADHD-I trios, but not ADHD-C	Non-significant
HTR1B A-161T	A/T		allelic TDT P-value=1.00, McNemar X²=0.00 in ADHD...... allelic TDT P-value=1.00, McNemar X²=0.00 in ADHD; allelic TDT P-value=0.583, McNemar X²=0.302 in ADHD combined type; allelic TDT P-value=0.671, McNemar X²=0.180 in ADHD inattentive type More...	no biased transmission was observed	Non-significant

Genes reported by this study (count: 1)